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Image Search Results
Journal: RSC Advances
Article Title: Polydopamine-functionalized acellular fish scale scaffolds for accelerated bone tissue regeneration
doi: 10.1039/d5ra01932j
Figure Lengend Snippet: Osteogenic differentiation ability of scaffolds in vitro . (A) ALP staining and ALP activity of BMSCs after 7 and 14 days of osteogenic induction, and ARS staining and quantitative analysis of calcium nodules after 21 days of osteogenic induction. Scale bar = 200 μm. (B and C) Fluorescence microscopy images and quantitative analysis of IF staining of RUNX2, OPN, and COL-1α in BMSCs after 14 days of osteogenic induction. Scale bar = 100 μm. (D) The mRNA expression levels of RUNX2, OPN, and COL-1α in BMSCs after 14 days of osteogenic induction. All values are presented as means ± standard deviation (ns: no significant differences, *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001).
Article Snippet: After 21 days of osteogenic induction, cells were fixed with 4% paraformaldehyde and stained using an
Techniques: In Vitro, Staining, Activity Assay, Fluorescence, Microscopy, Expressing, Standard Deviation